ABSTRACT
OBJECTIVE:To establish a GC-MS method to identify the volatile constituents in Radix Isatidis.METHODS:The method of wet distillation was applied to extracting volatile oil from Radix Isatidis,the chromatographic column was OV-1,and the flow rate was 1.0mL?min-1,EI ion source was used by MS.The volatile constituents in Radix Isatidis were identified and analyzed by NIS107 system.RESULTS:22 chromatographic peaks were separated,19 chemical compounds were identified,representing 90.51% of total amount,hexadecanoic acid had highest content,accounting for 38.52% of total amount.CONCLUSION:This experiment has provided initial usable data for comprehensive utilization of Radix Isatidis.
ABSTRACT
Objective To establish the standard of quality control for Pericarpium Citri Reticulatae Viride (PCRV) and to identify two kinds of PCRV which are collected in different times. Methods In this article,volatile components and flavonoid in 10 batches of samples which were collected from eight provinces were determined by GC and HPLC-DAD,respectively. Their fingerprints were handled by weighted similarity of range and by principal component analysis (PCA). Results According to the result of similarity and classification,there were obvious differences between the two kinds of PCRV.To know whether the differences have great influences on the pharmacological actions of PCRV, pharmacological studies will be needed. Weighted similarity is suitable for this system which has a high content of similar components. Conclusion These methods are effective and will help us to establish the criterion of quality control for PCRV.
ABSTRACT
Objective To compare the difference in constituents from different parts of wild and cultivated Ephedra sinica. Methods Using the solvent of 50% methyl alcohol to extract from the stems and roots of wild and cultivated E. sinica under heating reflux, respectively and then determine the content of ephedrine in each extractions by HPLC. The volatile oil was extracted by stream-distillation and analyzed by GC-MS method. Results The content of the ephedrine in extraction of stems and roots of wild E. sinica was 0.55% and 0.000 57% while the content of the cultivated was 0.26% and 0.001 7%. Forty-five compounds in the volatile oil were identified by GC-MS method, among them 13 compounds existed in both wild and cultivated E. sinica. Conclusion The content of the ephedrine is about two times of the cultivated in wild E. sinica′s stems, while it is very low in the roots of two kinds of herbs. And ephedrine is not found in the volatile oil.